Contact an Account ManagerAn UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues Solid Phase Extraction
Summary
Type: SPE
Application #: 305620
Detection:
Conditions: SPE was performed in 96-well plate format using CHROMABOND® MULTI 96 HR-XC plates and deep well receiving plates (e.g., 96-deep-well collecting plates (polypropylene) 96 x 2 mL, REF 738650.5) in conjunction with centrifugation.
Conditioning: 1 mL methanol, then 1 mL water
Sample aspiration: pretreated plant extracts were loaded on each well in 5 x 1 mL-portions
Elution: 900 µL acetonitrile
Storage: 850 µL of the eluate fraction were transferred to a 1.5-mL polypropylene tube and stored at -20 °C before LC-MS. Alternatively, eluate fractions were completely dried before storage.
Authors: Balcke, G.U. et al.
Source: Plant Methods 2012, 8: 47
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